5-Amino-1-methylquinolinium (5-Amino-1MQ) is a small molecule inhibitor of nicotinamide N-methyltransferase (NNMT) β an enzyme that methylates nicotinamide (niacin), consuming S-adenosyl methionine (SAM) in the process. By inhibiting NNMT, 5-Amino-1MQ raises NAD+ precursor availability and alters fat cell metabolism, making it a research tool for obesity, adipogenesis, and metabolic syndrome studies.
CAS 1073485-20-7 | MW 192.2 DaBlocks nicotinamide N-methyltransferase, preventing conversion of NAM to MNA and increasing NAD+ precursor (NAM) availability in adipocytes.
NNMT inhibition reduces PPAR-Ξ³-driven adipocyte differentiation and decreases lipid accumulation in 3T3-L1 preadipocyte research models.
Reducing NNMT activity conserves SAM for methylation reactions, altering epigenetic methylation patterns in fat tissue.
Elevated NAM availability increases SIRT1 activation via NAD+ β connecting NNMT inhibition to longevity and mitochondrial research.
Parsons et al. (2016) demonstrated that 5-Amino-1MQ reduced fat cell size by up to 30% in isolated adipocytes and attenuated diet-induced obesity in mouse models without toxicity.
Cell Chem Biol. 2016;23(4):543β554NNMT knockout and inhibition studies both show increased energy expenditure and reduced adiposity, confirming 5-Amino-1MQ mechanism in metabolic research.
Science. 2014;343(6177):1296β1300Elevated NNMT in white adipose tissue of obese humans correlates with metabolic dysfunction β establishing NNMT as a validated obesity research target for 5-Amino-1MQ studies.
Nat Commun. 2015;6:6745Murine studies: 10β40 mg/kg/day orally. In vitro adipocyte studies: 1β50 Β΅M. Endpoints include lipid staining (Oil Red O), adipocyte diameter measurement, NNMT enzymatic assay, SAM/SAH ratio, and NAM levels. Often studied alongside NAD+ supplementation for comprehensive metabolic pathway research.
| Full Name | 5-Amino-1-methylquinolinium (5-Amino-1MQ) |
| CAS Number | 1073485-20-7 |
| Molecular Formula | CββHββNβ |
| Molecular Weight | 192.2 Da |
| Target | NNMT (nicotinamide N-methyltransferase) |
| Purity | β₯99% by HPLC |
| Appearance | Off-white crystalline powder |
| Storage | β20Β°C, desiccated |
